Keywords: Metformin, RP-HPLC, ICH Guidelines Proposed method can be adoptable for quality control analysis of Metformin. Linearity, accuracy, precision, inter-day and intra-day variation were checked,Īssay studies of Metformin were within 98% to 102% indicating that the The statistical validation parameters such as Methanol was used as a mobile phase with various ratios and flow rates,Įventually 30:70 v/v phosphate buffer with pH of 3.0 and Methanol was being Isocratic elution of phosphate buffer with pH of 3.0 and Was separated on C18 column, usingĬombination of phosphate buffer with pH of 3.0 and Methanol at the UVĭetection of 238nm. Reverse PhaseHigh PerformanceLiquidChromatography(RP-HPLC).Metformin Pawar, College of Pharmacy, Manur, Kalwan, Maharashtra, IndiaĪ simple and reproducible method was developed for Metformin (MET) by Prashant Malpure4ġ,3Sandip Foundation, Institute of Pharmacy, Mahiravani, Nashik, Maharashtra, IndiaĢ,4Loknete J. Hydrochloride by using RP-HPLC with ICH Guidelines Volume: 3 | Issue: 3 | Mar-Apr 2019 Available Online: e-ISSN: 2456 - IJTSRD | Unique Paper ID - IJTSRD22812 | Volume – 3 | Issue – 3 | Mar-Apr 2019 Page: 415Īnalytical Method Development and Validation of Metformin International Journal of Trend in Scientific Research and Development (IJTSRD) Prashant Malpure ''Analytical Method Development and Validation of Metformin Hydrochloride by using RP-HPLC with ICH Guidelines'' Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-3 | Issue-3, April 2019, URL: Īnalytical Method Development and Validation of Metformin Hydrochloride by using RP HPLC with ICH Guidelines The statistical validation parameters such as linearity, accuracy, precision, inter day and intra day variation were checked, assay studies of Metformin were within 98 to 102 indicating that the proposed method can be adoptable for quality control analysis of Metformin. Isocratic elution of phosphate buffer with pH of 3.0 and Methanol was used as a mobile phase with various ratios and flow rates, eventually 30 70 v v phosphate buffer with pH of 3.0 and Methanol was being set with the flow rate of 1mL min.

Metformin was separated on C18 column 4.6x250mm, particle size 5Âµm, using combination of phosphate buffer with pH of 3.0 and Methanol at the UV detection of 238nm. A simple and reproducible method was developed for Metformin MET by Reverse Phase High Performance Liquid Chromatography RP HPLC.

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